Effects of zinc on expression of apoptosis-related genes in freezing thawing damage of adipose tissue derived mesenchymal stromal cells.
Nesari F., Gholami M., Rezaian J., Pirnia A., Anbari K., Beigi Boroujeni M.
Animal Study, published in Prep Biochem Biotechnol (2021) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Prep Biochem Biotechnol (2021)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 34694192
- DOI
- 10.1080/10826068.2021.1983830
Abstract (original English)
The present study was performed to investigate the effects of zinc supplementation on freezing thawing damage in adipose tissue-derived mesenchymal stromal cells (MSC) of mice through studying cellular viability and gene expression profile of apoptosis. Slow freezing method was conducted and the samples were treated with zinc doses 0, 2.5, 5, 10, 25, 50 and 100 µM. Viability was increased in groups of 2.5, 10 and 25 µM zinc in comparison to the control group. Gene expression study showed that in the group of 2.5 µM zinc, Fas , Bax and Caspase3 had down regulation. Up regulation of Bcl2 was observed in the groups of 10 and 25 µM zinc. P53 did not have a protecting regulation in the groups of study. The present study showed that doses 2.5-25 µM of zinc had a rather safe toxicity, increased cellular viability, and ameliorated expression of apoptosis-related genes in both intrinsic and extrinsic pathways.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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