Efficacy of Human Adipose-Derived Stem Cells in Spinal Fusion in a Rat Ovariectomy Osteoporosis Model.

Several human studies show positive signals, while research methods and sample sizes continue to develop.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Aim To evaluate the efficacy of human adipose-derived stem cells (h-ADSCs) in spinal fusion in an osteoporotic rat model. Material and methods Female Sprague-Dawley rats (n=40) underwent ovariectomy and were then randomly assigned into two groups: ovariectomy (OVX) (OVX + fusion) and h-ADSCs (OVX + fusion + h-ADSCs). Six weeks after OVX, we performed bilateral lumbar spinal fusion using the autologous iliac bone with or without administration of h-ADSCs. The efficacy of the spinal fusion was then assessed using manual palpation, lateral ending, morphogenic examinations, and histology six weeks the after fusion procedure. Results Fusion bed volume was different between the two groups but not significantly. However, the fusion bed density was higher in the h-ADSC group than in the OVX group. Manual palpation (70% vs. 40%, p=0.112) and lateral bending (95% vs. 55%, p=0.011) produced higher fusion rates in the h-ADSC group than in the OVX group. Additionally, a histologic examination revealed new bone formation at the fusion bed between the lamina and implanted iliac crest bone in the h-ADSC group, whereas, in the OVX group, the fusion masses were composed of fibroblastic proliferation. Conclusion Our study demonstrates that the administration of h-ADSCs may have advantages in bone formation and consolidation but does not lead to bone overgrowth. These findings indicate that the ad

Evidence level

Several human studies show positive signals, while research methods and sample sizes continue to develop.

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