Efficacy of vocal fold injection of dedifferentiated fat cells in treating glottis closure insufficiency: Insights from a rat model of recurrent laryngeal nerve resection
Mikoshiba I., Hagikura K., Matsumoto T., Kazama T., Asai R., Hao H.
Animal Study, published in PLoS One (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- PLoS One (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 40911610
- PMCID
- PMC12412957
- DOI
- 10.1371/journal.pone.0324965
- Citations
- 1
Abstract (original English)
Glottic insufficiency results from impaired vocal fold contact, leading to a gap between the folds and manifesting as hoarseness and respiratory difficulties. Vocal folds injection is a commonly utilized therapeutic approach to rectify this gap by augmenting vocal folds volume; however, the optimal injectable material remains undetermined. Dedifferentiated fat cells (DFATs), derived from mature adipocytes, exhibit robust proliferative capacity and multipotency, establishing them as potential candidates for treating glottic insufficiency. This study investigated the therapeutic efficacy of DFATs in a rat model of recurrent laryngeal nerve paralysis. Twenty-five rats were used for the therapeutic evaluation: the recurrent laryngeal nerve of each rat was resected unilaterally, and 5 weeks later, the atrophied vocal fold muscles were injected with either 10 μl of saline (control), 1.0 × 106 DFATs in 10 μl (DFAT group), or 1.0 × 106 DFATs combined with 500 ng of basic fibroblast growth factor (bFGF) in 10 μl (DFAT + bFGF group). At 4 weeks post-injection, laryngeal endoscopy evaluated the glottic gap, followed by histological analyses of muscle atrophy, collagen deposition. To investigate mechanisms, an independent cohort of 25 rats received identical treatments and was evaluated 2 weeks post-injection for cell proliferation (Ki-67) and apoptosis (TUNEL). To confirm DFAT engraftment
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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