The engineered bladder patch with a three-layer structure promotes the regeneration and functional recovery of the bladder in a rabbit model.
Li D., Wang P., Bi K., He Y., Zhang J., An Z.
Laboratory Study, published in Bioeng Transl Med (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Bioeng Transl Med (2026)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42394900
- DOI
- 10.1002/btm2.70140
Abstract (original English)
The engineered bladder patch with a three-layer structure was constructed from a bi-layer silk fibroin scaffold (BSFS) and a methacrylated bladder acellular matrix hydrogel (BAMMAH) that carried induced vascular endothelial cells (VECs) and induced nerve cells (NCs). The patch was developed for bladder augmentation in a rabbit model. The prepared BSFS combined the rigidity of silk fibroin film with the elasticity of silk fibroin sponge, providing a support framework for the construction of engineered bladder. The synthesized BAMMAH exhibited a rapid gelling property, and 2% BAMMAH showed good rheological properties and an internal pore structure. Immunofluorescent staining and RT-qPCR confirmed that the induction schemes for adipose-derived mesenchymal stem cells (ADSCs) differentiation into VECs and NCs were feasible and stable. Immunofluorescence analysis demonstrated that incubation with omentum could promote the regeneration of the vascular network inside the engineered patch with a three-layer structure (BSFS-BAMMAH-ADSCs-VECs-NCs). Animal experimental data showed that the engineered patch could promote the regeneration of the bladder wall and the recovery of bladder function. These results confirmed that the constructed engineered bladder patch with a three-layer structure could regenerate the blood vessel network and neural reinnervation, providing a feasible method to s
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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