Engineering Resilience: How Irradiation Strategies Influence 3D-Bioprinted Adipose Stem Cells.
Amiri N., Schmid R., Schrüfer S., Lamberger Z., Stahlhut P., Lang G.
Laboratory Study, published in Bioengineering (Basel) (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Bioengineering (Basel) (2025)
- Country
- Switzerland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41595957
- PMCID
- PMC12837484
- DOI
- 10.3390/bioengineering13010025
Abstract (original English)
Background Reconstructive defect coverage after irradiation remains a challenge in reconstructive surgery, as ionizing radiation leads to tissue ischemia and fibrosis. Therefore, the application of adipose-derived stem cells (ASCs) might be a therapeutic strategy for improving flap survival. Nevertheless, the influence of irradiation on ASCs remains unclear. This study examines the effect of irradiation on 3D-printed ASCs. Methods ASCs were 3D-cultured. The constructs were irradiated with 2 Gy and 5 Gy; one group treated with 0 Gy served as the non-irradiated control group. Cell viability was measured via a WST-8 assay, a live/dead assay and fluorescence microscopy 24 h, 48 h and 72 h after irradiation. Furthermore, qPCR analysis was performed to detect the expression of HIF-1α, p53 and IL-6 at the same timepoints. Results Cell survival was high after 24 h. Expression of HIF1α after 24 h was 1.5 times significantly higher in the 2 Gy group compared with the 0 Gy group. The expression of other genes was not significantly affected by irradiation. Measurement of the metabolic activity and survival of the ASCs did not show differences between the different groups at all timepoints. Conclusions 3D-cultured adipose-derived stem cells maintain high viability after moderate irradiation, suggesting radioresistance.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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