Enhanced chondrogenesis of adipose-derived stem cells by the controlled release of transforming growth factor-beta1 from hybrid microspheres.
Han Y., Wei Y., Wang S., Song Y.
Animal Study, published in Gerontology (2009) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Gerontology (2009)
- Country
- Switzerland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 19672054
- DOI
- 10.1159/000235547
- Citations
- 20
Abstract (original English)
Background Articular cartilage has a limited self-regenerative capacity, and tissue engineering is a promising solution to the problem of cartilage damage. Objective The aim of this study was to evaluate the effect of hybrid microspheres (MS) composed of transforming growth factor (TGF)-beta1-loaded gelatin MS and chitosan MS on enhancement of the differentiation of adipose-derived stem cells (ASCs) into chondrocytes in pellet culture. Methods In vitro TGF-beta1 release was evaluated by an enzyme-linked immunosorbent assay. The content of DNA and glycosaminoglycans (GAGs) was tested by biochemical methods. In addition, quantitative PCR was used to analyze the expression of collagen II and aggrecan. Results Increased proliferation of ASCs was observed in the hybrid TGF-beta1-loaded MS in comparison to the TGF-beta1-loaded gelatin MS. The chondrogenic differentiation of ASCs in both constructs was evaluated, and GAG content and the gene expression of collagen II and aggrecan were significantly higher in the hybrid TGF-beta1-loaded MS than in the TGF-beta1-loaded gelatin MS. Conclusions Enhanced differentiation of ASCs by hybrid TGF-beta1-loaded MS may provide an easy and effective way to construct tissue-engineered cartilage.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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