Enhancing adipogenesis in Wharton's jelly multipotent mesenchymal stromal cells through lipidomic insights and fatty acid supplementation.
Rogulska O., Vavrinova E., Vackova I., Smolkova K., Petrenko Y.
Laboratory Study, published in Sci Rep (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Sci Rep (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40847078
- PMCID
- PMC12373758
- DOI
- 10.1038/s41598-025-16867-9
Abstract (original English)
Wharton's Jelly multipotent mesenchymal stromal cells (WJ-MSCs) hold potential for regenerative medicine, particularly in soft tissue engineering. However, their adipogenic differentiation capacity is inferior to adipose tissue-derived MSCs (AT-MSCs). This study aimed to optimize adipogenic differentiation for WJ-MSCs by leveraging insights from the comparative analysis of WJ- and AT-MSC lipidomic profiles. Lipidomic profiles of non-induced cells were compared, and adipogenic differentiation was induced with and without exogenous oleic or linoleic acid supplementation. Differentiation efficiency was determined based on lipid droplet formation, triglyceride (TG) content quantification, and the expression of adipogenic markers. Significant differences in TG composition were observed, with WJ-MSCs showing higher levels of 52-carbon TGs and AT-MSCs having more 56-carbon species. Both cell types had similar fatty acid (FA) profiles, with 18-carbon FAs making up over 50%. Adding oleic acid to the differentiation medium significantly enhanced lipid droplet formation and upregulated adipogenic markers in WJ-MSCs, aligning their adipogenic capacity more closely with AT-MSCs. In contrast, linoleic acid showed no significant benefits. The study underscores the critical role of the initial lipidomic profile in the adipogenic differentiation of MSCs. Supplementation with oleic acid represen
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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