Enhancing Tendon Regeneration: Investigating the Impact of Topography on the Secretome of Adipose-Derived Stem Cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Abstract Background : Tendons are vital for maintaining integrity and movement, but current treatment options are insufficient for restoring them after injuries. Previous studies have shown that the secretome from mesenchymal stem cells (MSCs) promoted tendon regeneration. However, limited studies have explored the impact of the cellular microenvironment on the secretome of MSCs in tendon repair. This study aims to investigate how the topographic orientation impacts the secretome of human adipose-derived stem cells (ADSCs) and its effect on tendon repair and regeneration. Methods: Randomly oriented and aligned silk scaffolds were prepared by directional freeze-drying. Conditioned medium (CM) was generated from ADSCs cultured on the scaffolds with different topography (RCM: random scaffolds; ACM: aligned scaffolds). In vitro experiments were performed to access the effect of RCM and ACM on cell proliferation by live/dead staining, CCK-8 incubation, and Ki67 immunofluorescence. The effect on tenogenic differentiation of tendon stem/progenitor cells (TSPCs) and polarization of macrophages was confirmed by detecting the expression of related genes. Subsequently, RCM and ACM were injected into rats with patellar tendon defects. Tissue repair and immunomodulatory effects were evaluated through histological and immunohistochemical staining. Result: In vitro results showed that the ACM

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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