Establishment of a bipotent cell line CL-1 which differentiates into chondrocytes and adipocytes from adult mouse.
Kitamura H.
Animal Study, published in Osteoarthritis Cartilage (2004) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Osteoarthritis Cartilage (2004)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 14697680
- DOI
- 10.1016/j.joca.2003.09.002
Abstract (original English)
Establishment of a clonal bipotent chondroprogenitor cell line from adult mouse to provide a new tool for the elucidation of chondrogenesis in adult animal. A clonal cell line CL-1 was established from tibia of adult mouse. Differentiation of CL-1 was characterized in monolayer culture. Effects of growth factors (TGF-beta(1), IGF-I, bFGF) and hormones (all trans retinoic acid, 1 alpha.25(OH)(2)D(3), PTH (1-34)) on the growth and differentiation of CL-1 were examined. Bipotency of CL-1 in vivo was examined by transplantation into SCID mice. CL-1 formed alcian blue (pH1.0) positive nodules spontaneously. The nodules were mineralized in the presence of ascorbic acid and beta-glycerophosphate. CL-1 differentiated also into oil red O positive adipocytes spontaneously. CL-1 cells expressed specific genes of chondrocytes (collagen type II, X, aggrecan) and adipocytes (PPAR-gamma(2), aP(2)). Hyaline cartilage and adipose tissue formation was observed also in subcutaneously transplanted CL-1 cells into SCID mice. These data demonstrate that CL-1 has bipotency either in vitro or in vivo. TGF-beta(1)suppressed growth of CL-1 and induced dominant chondrogenesis accompanied with marked suppression of adipogenesis in 10% FCS. IGF-I stimulated both growth (in 3% FCS) and differentiation of CL-1 into both lineages (in 10% FCS). 1 alpha.25(OH)(2)D(3)and all trans retinoic acid acted as negative
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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