Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root-Propolis Extract on HEK293T Cells
Marcinčáková D., Hudáková N., Miłek M., Kolesárová M., Dżugan M., Cizkova D.
Laboratory Study, published in Pharmaceuticals (Basel) (2024) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Pharmaceuticals (Basel) (2024)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 39861090
- PMCID
- PMC11769209
- DOI
- 10.3390/ph18010027
- Citations
- 3
Abstract (original English)
Background/Objectives: The health benefits of honeybee products and herbs are well known, and their appropriate combination may enhance their biological efficacy. This study investigated the biological properties of a combined barberry root and propolis extract (PBE) in comparison to a propolis extract (PE), a barberry root extract (BE), and pure berberine (BN). Methods: The antioxidant properties were evaluated using DPPH and FRAP methods and total phenolic contents (TPC) were assessed by the Folin-Ciocalteu method. HPTLC was used to quantify the BE in the tested samples. Their effect on HEK293T cells was monitored in real-time by using the xCELLigence system which recorded changes in the proliferative activity (PA). The metabolic activity (MA) was evaluated using an MTS test and cell migration was analyzed via a scratch assay. Results: The PE exhibited a higher TPC (198.67 mg/g) than the BE (119.3 mg/g). The PBE exhibited a comparable antioxidant effect to that of the PE. In the cell assays, the PE, the BE, and BN significantly reduced the proliferative activity at higher concentrations ( p 50 value was determined for the PBE (130 µg/mL), suggesting that this combination has a reduced cytotoxicity. However, the scratch test did not confirm a significant supportive effect of the PBE on cell migration. Conclusions: Although the PBE did not show enhanced antioxidant properties,
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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