Evaluation of Elaeagnus angustifolia extract in promoting osteogenesis of adipose-derived mesenchymal stem cells through modulation of intracellular signaling pathways.
Wang B., Zhou Z., Dai P., Pan Y., Ge J.
Animal Study, published in Tissue Cell (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Tissue Cell (2026)
- Country
- Scotland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41797242
- DOI
- 10.1016/j.tice.2026.103432
Abstract (original English)
Background Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates for bone tissue engineering; however, the limited bioactivity of synthetic scaffolds such as polycaprolactone (PCL) restricts their osteoinductive potential. Incorporation of natural plant-derived bioactives may enhance scaffold functionality. Objective This study investigated whether incorporation of Elaeagnus angustifolia extract into electrospun PCL scaffolds could enhance osteogenic differentiation of ADSCs in vitro. Methods Electrospun PCL scaffolds were fabricated and modified by overnight immersion in E. angustifolia extract. ADSCs were cultured on tissue culture polystyrene (TCPS), unmodified PCL, and extract-enriched PCL scaffolds. Cell viability was evaluated using MTT assay, while osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity, calcium deposition, and expression of osteogenic genes (RUNX2, COL1, ON, and OC). Results No significant differences in cell viability were observed among the experimental groups, indicating good cytocompatibility. In contrast, ADSCs cultured on E. angustifolia-enriched PCL scaffolds exhibited significantly increased ALP activity, enhanced calcium deposition, and upregulated expression of osteogenic marker genes compared to unmodified PCL and TCPS. Conclusion Incorporation of E. angustifolia extract into PCL scaffolds enhances ost
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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