Evaluation of Platelet Lysate-Based Medium and Protein Substrate for HUVEC Culture and Expansion
Duarte Rojas JM., Restrepo Múnera LM., Estrada Mira S.
Laboratory Study, published in Biomedicines (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Biomedicines (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 40427014
- PMCID
- PMC12109029
- DOI
- 10.3390/biomedicines13051187
Abstract (original English)
Background/Objectives: Endothelial cell (EC) culture relies on specialized and commercial media with distinct growth supplement compositions. These media are expensive and must be imported, increasing the time to effective use. Human platelet lysate (PL) and platelet lysate serum (PLS) supplemented media are emerging alternatives to commercial media. Methods : Umbilical cords were collected, and human umbilical vein endothelial cells (HUVEC) were isolated and cultured using different media formulations, using Endothelial Cell Growth, Promocell ® (ECGM-Promocell ® ) commercial medium, and media supplemented with PL and PLS. Results: A mixed medium combining DMEM-F12 + PLS and ECGM- Promocell ® maintained EC viability, adhesion, and proliferation. Introducing a PL-derived protein substrate enhanced cell adhesion and proliferation by simulating an extracellular matrix. Flow cytometry revealed positive CD31, CD144, and CD146 markers in cells cultured with ECGM- Promocell ® and the mixed medium, with or without the PL-protein substrate. Conclusions: These findings suggest that the mixed medium, especially with the PL protein substrate, offers a cost-effective and efficient approach for EC culture and proliferation, holding promise for research and therapeutic applications.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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