[Experiment of adipose derived stem cells induced into smooth muscle cells].
Yang P., Yin S., Cui L., Li H., Wu Y., Liu W.
Laboratory Study, published in Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2008) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2008)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 18575454
- Citations
- 4
Abstract (original English)
To study the feasibility of human adipose derived stem cells (ADSCs) in monolayer culture induced into smooth muscle cells in vitro as seeding cells in vascular tissue engineering. The mononuclear cells in human adipose were separated by collagenase treatment and seeded on culture dishes with the density of 5 x 10(5)/cm2. Cells were cultured in M-199 plus 10% FBS. When reaching confluence, the cells were subcultured by 0.1% trypsin and 0.02% EDTA treatment, PDGF-BB (50 ng/mL) and TGF-beta1 (5 ng/mL) were added at the passage 1 to enhance the smooth muscle cells' phenotype. Cells were cultured under the inducing medium for 14 days. The morphology of induced cells was observed under the microscope. Cellular immunofluorescence and RT-PCR were used to determine the expression of smooth muscle cell markers of the post-induced cells. Flow cytometry (FACs) was used to examine the positive rate of induced team. Cocultured in M-199 media including TGF-beta1 and PDGF-BB, the proliferating capability of the induced cells was significantly downregulated compared with the uninduced cells (P < 0.01). The induced cells exhibited "Hill and Valley" morphology, while the uninduced cells were similar to ADSCs of P0 which had the fibroblast-like morphology. The results of immunofluorescence indicated that the induced cells expressed smoothmuscle (SM) cell-specific markers including a-smooth muscle
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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