[Experimental expression of HIF-1α in rabbit adipose-derived mesenchymal stem cells by dynamic compression plus IGF-1 gene transfection].
Yang J., Zhang CH., Li JJ., Zhou ZD., Zhang QM.
Animal Study, published in Zhonghua Yi Xue Za Zhi (2010) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Zhonghua Yi Xue Za Zhi (2010)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 21223773
- DOI
- 10.3760/cma.j.issn.0376-2491.2010.45.015
- Citations
- 2
Abstract (original English)
To investigate the effects on HIF-1α expression of rabbit adipose-derived mesenchymal stem cells (ADSCs) by dynamic compression plus IGF-1 gene transfection and explore the mechanism of promoting chondrogenesis by HIF-1α. The ADSCs were harvested after the digestion of typeIcollagenase and transfection with pcDNA3.1-IGF-1. And then the cells were seeded onto chitosan/gelatin scaffolds with a density of 5 × 10(7) cells/ml and divided into groups: Group A (control), non-transfected ADSCs; Group B (IGF-1), hIGF-1 gene transfected ADSCs; Group C (loading), untransfected ADSCs with stimulation of compressive loading; Group D (loading + IGF-1), hIGF-1 gene transfected ADSCs and loading stimulation. The dynamic compression was carried out with a bio-reactor at a frequency of 0.1 Hz and a sinusoidal strain amplitude of 2% (2% at 0.1 Hz). The dynamic load was performed every 20 minutes, 4 hours daily. After 7 days, morphological observation was performed. The MTT assay was used to detect the cell proliferation. Meanwhile the CM-DiI cell-labeling solution was used to observe the distribution of cells. The total amount of GAG and the expression of related genes of IGF-1, HIF-1α, type II collagen (COL II) and Sox-9 were quantified. The best morphology was found in loading+ IGF-1 group. The results of proliferating capacity were: Group A < C < B < D (P < 0.01); Dil fluorescence showed that
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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