[Experimental research of the promotion effect of autogeneic PRP on osteogenic differentiation of human adipose-derived stem cells in vitro].
Li H., Liu D., Yu Y., Wu T.
Prospective Study, published in Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2009) — summary generated from the PubMed abstract.
Early human evidence such as case series or small samples is exploring possible benefits.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Prospective Study
- Journal
- Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2009)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 19594024
- Citations
- 4
Abstract (original English)
To study the effect of autogeneic PRP on proliferation and osteogenetic differentiation of human adipose-derived stem cells (ADSCs) in vitro. ADSCs were isolated from adipose tissue obtained from donor undergoing liposuction and were cultured, and growth condition of the cells was observed by inverted microscope. ADSCs at passage 3 were cultured in adipogenic or chondrogenic medium and underwent identification, immunofluorescence staining observations for CD29 and CD44 were performed. ADSCs at passage 3 were divided into 2 groups: PRP group cultured by osteogenic induction culture medium containing 10 mL/L PRP, and control group cultured by osteogenic induction culture medium without PRP. Then growth condition of the cells was observed by inverted microscope. MTT method was used to observe cell proliferation activity 1, 2, 3, 4 and 5 days after culture. ALP activity detection was conducted 7, 14, 21 and 28 days after culture. ALP staining was performed on PRP group 7 and 14 days after culture. Alizarin red staining was performed on PRP group 14 days after culture to detect the formation of calcium nodule. Under the inverted microscope, most ADSCs at passage 3 were spindle-shaped and the doubling time was about 35 hours. Adipogenic and chondrogenic differentiation were confirmed, and the cells were positive for CD29 and CD44 immunofluorescence staining. MTT method revealed the a
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
Evidence level
Early human evidence such as case series or small samples is exploring possible benefits.
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