[Experimental study of adipose tissue differentiation using adipose-derived stem cells harvested from GFP transgenic mice].
Lu F., Gao JH., Mizuro H., Ogawa R., Hyakusoku H.
Animal Study, published in Zhonghua Zheng Xing Wai Ke Za Zhi (2007) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Zhonghua Zheng Xing Wai Ke Za Zhi (2007)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 18161358
- Citations
- 3
Abstract (original English)
Objective To identify the expression of protein which is characteristic of stem cell, induce the adipogenic differentiation in vitro by ASCs harvested from GFP transgenic mice, and to assess the possibility of constructing adipose tissue via the attachment of ASCs to type I collagen scaffold. Methods Inguinal fat pads from GFP transgenic mice were used for the isolation of ASCs. Expression of CD29, CD34, CD45, CD133 and HLA-DR were detected by flow cytometry. After expansion to three passages, the ASCs were incubated in an adipogenic medium for two weeks. Then they were attached to collagen I scaffold and co-cultured for 12 hours, followed by transplantation under the dorsal skin of athymic mice for 2 months. Adipogenic differentiation of ASCs in vitro was assessed by morphological observation, Oil red O staining and newly formed tissue was detected by HE staining. Results The cultured stem cells were fibroblast-like cells and showed highly homogeneous appearance with active proliferation capacity. Stem cells' characteristic CD expression was proved. After being incubated in an adipogenic medium, they could differentiate into mature adipocytes. Accumulation of lipid droplets in the cytoplasm was testified by Oil red O staining, morphological and biological observation. 0.5 ml new tissue was formed and was confirmed by fluorescent observation and HE staining to be mature adipose
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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