Exploring the differentiation potential of adipose tissue-derived mesenchymal stromal/stem cells and progenitor buccal epithelial cells into urothelial cells.
Buhl M., Jundziłł A., Dąbrowski P., Szeliski K., Rasmus M., Kloskowski T.
Prospective Study with a reported sample of 5, published in Front Bioeng Biotechnol (2025) — summary generated from the PubMed abstract.
Early human evidence such as case series or small samples is exploring possible benefits.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Prospective Study
- Journal
- Front Bioeng Biotechnol (2025)
- Country
- Switzerland
- Reported sample size
- 5
- Source database
- PubMed
- PMID
- 41602467
- DOI
- 10.3389/fbioe.2025.1687541
Abstract (original English)
The intestinal wall is the most commonly used substitution material in reconstructive urology. However, this strategy is associated with many complications. Tissue engineering techniques can replace the intestinal wall with an in vitro-created urinary tract substitute. Biomaterial seeding with urothelial cells (UCs) would increase the success of the regeneration process. The problem in many patients requiring urinary reconstruction is the lack of autologous UCs. This study aimed to compare the differentiation potential of porcine mesenchymal adipose tissue-derived stromal/stem cells (AD-MSCs) and progenitor buccal epithelial cells (pBECs) toward UCs. UCs, AD-MSCs, and BECs were isolated from tissues harvested from domestic pigs. The conditioned medium was collected from UC cultures (UC-CM) between the first and third passage (n = 5). AD-MSC and BEC cultures were exposed to UC-CM for 7 and 14 days, respectively. UCs from the third passage (day 0) were used as a positive control. The differentiation effect of UC-CM was characterized by analysis of selected UC-typical markers at the mRNA and protein levels. AD-MSCs subjected to urothelial differentiation changed their morphology from fibroblast-like to epithelium-like. In BECs, the differentiation factor increased the expression of urothelial markers, including CK8 , UPK1B , and UPKIII. This study demonstrated that pBECs have a hi
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
Evidence level
Early human evidence such as case series or small samples is exploring possible benefits.
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