Expression of insulin-like growth factor type 1 receptor and myosin heavy chain in rabbit's rotator cuff muscle after injection of adipose-derived stem cell.

Several human studies show positive signals, while research methods and sample sizes continue to develop.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Purpose This study was performed to evaluate the effects of adipose-derived mesenchymal stem cells (ADMSC) in a subacute rotator cuff tear model of a rabbit by way of comparing the expression of insulin-like growth factor 1 receptor (IGF-1R) and myosin heavy chain (MyHC) in the ADMSC injected muscle and control. Methods Supraspinatus tears were created in both shoulders of 11 rabbits, and rotator cuff repair was performed after 3 weeks. At the time of repair, each side of the shoulders was randomly selected, and the injection of the ADMSCs at the muscle belly near musculotendinous junction (injection side) and saline to the contralateral side (control side) was performed. After 3 weeks, we randomly assigned 5 rabbits to the immunohistochemistric analysis and 6 to Western blot analysis. Results Expression of both IGF-1R (95 kD) and MyHC (200 kD) at the injection side was significantly elevated compared to control side (both p = 0.028). Immunohistochemistry showed that staining areas of both IGF-1R and MyHC were overlapped with staining of ADMSCs. Conclusion The injection of ADMSCs resulted in high expression of IGF-1R and MyHC in subacute rotator cuff tear and repaired model in rabbit compared to control side. Therefore, the injected ADMSCs may assist in regeneration of the rotator cuff muscle by way of insulin-like growth factor 1 (IGF-1) signalling pathway. This result may sug

Evidence level

Several human studies show positive signals, while research methods and sample sizes continue to develop.

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