Extracellular matrix components secreted by microvascular endothelial cells stimulate preadipocyte differentiation in vitro.
Varzaneh FE., Shillabeer G., Wong KL., Lau DC.
Animal Study, published in Metabolism (1994) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Metabolism (1994)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 8028517
- DOI
- 10.1016/0026-0495(94)90275-5
Abstract (original English)
Paracrine interaction between preadipocytes and microvascular endothelial cells may play a role in the regulation of adipose tissue growth. We report here a study of the effect of extracellular matrix factors secreted by microvascular endothelial cells, derived from adipose tissue, on preadipocyte differentiation in primary culture. Extracellular matrix components (EC) were prepared by differential centrifugation of medium conditioned by microvascular endothelial cells (CM). Preadipocyte differentiation was assessed by enumerating cells containing Oil-Red-O-stainable neutral lipids and by assaying cellular triacylglycerol (TG) content and glycerol-3-phosphate dehydrogenase (GPDH) specific activity. Both supernatant (containing soluble components) and pelleted (containing large complexes of EC) fractions of CM stimulated preadipocyte differentiation. When the supernatant fraction was used, the proportion of cells containing visible lipid droplets was 29% +/- 3% of total preadipocytes in the presence of extracellular complexes, as compared with 6% +/- 1% under control conditions. This differentiation induction was associated with fourfold increases in TG content and GPDH specific activity. Neither the supernatant nor the pelleted fraction of EC affected the maximal differentiation induced by hormonal stimulation in serum-supplemented or serum-free media. The major EC, fibronectin
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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