Extracellular matrix from decellularized porcine organs as scaffolds for insulin-secreting cells and pancreatic islets

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Extracellular Matrix (ECM) from different organs has been used to cultivate several cell types. ECM produced by organ decellularization contains collagens, fibronectin, glycosaminoglycans (GAGs), laminins and other components essential in providing structural support and biochemical cues for cells to attach, function, and proliferate. The organ from which ECM is extracted and produced is hypothesized to play a vital role in cell responses upon recellularization. To investigate this hypothesis, five porcine organs (bladders, kidneys, livers, lungs, and pancreas) were decellularized by a detergent-based method or by detergent-free procedures. Insulin-secreting rat pancreatic β-like cells (INS-1) were first used to screen, over 7 days, the effect of the ECM produced by the tested decellularization techniques from the five selected organs, revealing SDS treatment did not result in cell responsive ECMs for all the tested organs. Detergent-free-derived ECMs, on the other hand, allow cell attachment except for the pancreatic ECM. The biocompatibility of the ECMs made from detergent-free methods was subsequently validated using cell proliferation and cell metabolism assays, immunostaining for insulin and actin expression, as well as glucose-stimulated insulin secretion (GSIS). INS-1 cells proliferated on certain detergent-free ECMs and secreted insulin following 7 days of culture. Furt

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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