Extracellular vesicle-derived miR-511-3p from hypoxia preconditioned adipose mesenchymal stem cells ameliorates spinal cord injury through the TRAF6/S1P axis.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Extracellular vesicle (EV) from hypoxic adipose tissue-derived mesenchymal stem cells (AD-MSCs) play critical roles in spinal cord injury (SCI) by transferring miRNAs to target cells through fusion with the cell membrane. However, the role of miR-511-3p within the AD-MSCs -derived EV in SCI is largely unknown. Western blotting results demonstrated the secretion of EVs derived from AD-MSCs under hypoxia (Hyp-EVs) was more than those under normoxia (Nor-EVs), and miR-511-3p expression was more enriched in Hyp-EVs. PC12 cells were stimulated with lipopolysaccharide (LPS) to induce cell damage. AD-MSCs were transfected with miR-511-3p mimic or miR-511-3p inhibitor to induce EVs-miR-511-3p overexpression or silencing. Cells treated with Hyp-EVs-miR-511-3p mimic reduced LPS-induced apoptosis, alleviated inflammation and promoted proliferation, while cells treated with Hyp-EVs-miR-511-3p inhibitor aggravated LPS-induced apoptosis and inflammation, and suppressed proliferation. Luciferase reporter gene assay revealed tumor necrosis factor receptor-associated factor 6 (TRAF6) was a target downstream gene of miR-511-3p. A series of gain- and loss-of-function experiments verified that TRAF6 could antagonize the effects of Hyp-EVs-miR-511-3p on inflammation, cell apoptosis and viability. Furthermore, cells treated with CYM5541, an agonist of sphingosine-1-phosphate receptor 3 (S1PR3), reve

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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