Extracellular vesicles modulate skin aging biomarkers in a 3D reconstructed full-thickness skin model.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Extracellular vesicles (EVs) are lipid-enveloped nanovesicles rich in microRNAs, proteins and lipids, that serve as potent mediators of intercellular communication. While EVs have demonstrated pro-regenerative potential in 2D and preclinical models, their impact on skin regeneration and aging processes in 3D reconstructed skin models has remained less explored. In this study, EVs from adipose-derived stem cells and umbilical cord-derived mesenchymal stem cells (UC-MSCs) were evaluated using both 2D primary skin cells and 3D full-thickness reconstructed skin models. EVs stimulated fibroblast and keratinocyte proliferation, increased epidermal thickness, and enhanced the presence of collagen IV in the dermal-epidermal junction (DEJ) and fibrillin 1 in the extracellular matrix. Bulk transcriptomic analysis of the 3D reconstructed skin revealed gene expression profiles impacted by the addition of EVs. Additionally, miRNA-seq and proteomics of extracellular vesicle contents revealed miRNAs and proteins that may be drivers of the biological activities observed in 3D models, suggesting EVs activate processes associated with skin regeneration. This holistic approach demonstrated that EVs previously linked to pro-regenerative behaviors also modulate biomarkers associated with cutaneous aging in full-thickness 3D reconstructed models. This work not only provides mechanistic insights but

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

Browse all related research

Filter the research library by this study's title keywords, author, or publication year.