Extract of Bombycis Feces suppressed 3T3-L1 adipogenesis resulting in the regulation of fatty acid-dependent energy consumption

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

In Oriental medicine, silkworms and their derivatives have been used for anti-inflammatory and diabetic purposes. Bombycis Feces exhibit anti-atopic effects and anti-proliferative activity; however, the role of BF extract (BFE) in adipogenesis and obesity and management remains underexplored. This study examined the effects of BFE on amino acid oxidation during adipocyte differentiation. An adipogenesis model was established using 3T3-L1 cells. Intracellular lipid accumulation was assessed via Oil Red O staining, while cytotoxicity was evaluated using a cell viability assay. The effects of BFE on gene and protein expression, lipolysis, real-time oxygen consumption rate, and substrate oxidation during adipocyte differentiation were analyzed. The involvement of the branched-chain amino acid (BCAA) catabolic pathway and leptin gene expression was also evaluated. BFE treatment significantly downregulated the expression level of key adipogenic transcription factors, including PPARγ and C/EBPα, as well as aP2 gene expression. Lipid accumulation was significantly reduced, accompanied by increased expression of Sirt1 and Sirt6, but not Sirt3, and a concurrent reduction in Parp1 expression. In addition to adipogenesis, the oxygen consumption rate and extracellular acidification rate were downregulated by BFE treatment in adipocytes. Conversely, BFE enhanced amino acid-dependent oxidatio

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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