Factors Influencing SVF Yields from Human Adipose Tissue: Isolation Technique, Age, and Sex.
Regener S., Joy E., Comella K., Kim S.
Retrospective Study with a reported sample of 100, published in J Clin Med (2026) — summary generated from the PubMed abstract.
Early human evidence such as case series or small samples is exploring possible benefits.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Retrospective Study
- Journal
- J Clin Med (2026)
- Country
- Switzerland
- Reported sample size
- 100
- Source database
- PubMed
- PMID
- 41827467
- PMCID
- PMC12985714
- DOI
- 10.3390/jcm15052051
- Citations
- 1
Abstract (original English)
Background/Objectives: Stromal vascular fraction (SVF) from adipose tissue contains regenerative cell populations, including adipose-derived stem cells (ADSCs), and is increasingly used in clinical therapies. However, the effects of isolation technique and donor characteristics on SVF yield and viability remain unclear. This study aims to assess the impact of mechanical versus enzymatic isolation, as well as donor age and sex, on SVF total nucleated cell count (TNC) and viability. Methods: A retrospective analysis was conducted on 114 patients undergoing ADSC harvesting via a mini-liposuction. SVF was isolated using enzymatic digestion ( n = 100) or mechanical digestion ( n = 14). Percent viability and TNC were assessed using the Chemometec NC-200 NucleoCounter ® . The influence of isolation technique, donor age, and donor sex on SVF yield and viability was evaluated using Pearson's correlation and independent t -tests. Results: Enzymatic digestion yielded significantly higher cell viability compared to mechanical isolation ( p < 0.001), although no significant difference in TNC was observed between the two methods. Increasing donor age was modestly associated with reduced viability in enzymatically processed samples but not in mechanically processed samples. Donor age showed no significant association with TNC for either isolation method. Donor sex was not correlated with viab
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • Without an adequate control group, treatment effects cannot be separated from other factors.
Evidence level
Early human evidence such as case series or small samples is exploring possible benefits.
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