Fat harvesting protocol for enhanced stem cell viability - pilot study.
Pimenta R., Guimarães VR., Gomes SA., Altran SC., Sampaio ECDF., Tavares ER.
Laboratory Study, published in Clinics (Sao Paulo) (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Clinics (Sao Paulo) (2026)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42030687
- PMCID
- PMC13127207
- DOI
- 10.1016/j.clinsp.2026.100945
Abstract (original English)
Objective Adipose tissue is used in several specialties to obtain Adipose-derived Stem Cells (ASC) and fat derivatives. Several studies analyze protocols for harvesting fat tissues. However, these studies showed inconclusive data regarding cell viability and function. Thus, the objective of this study was to analyze protocols regarding cell viability, identify the best method, and test cell differentiation and proliferation when using the chosen methods. Methods This study harvested lipoaspirate products from three healthy women undergoing aesthetic procedures. The first analysis corresponds to the viability of fat tissue liposuction products for the washing process. The second analyzes the effect of anesthetic drugs, and the third analyzes the cytotoxicity of the fraction process. After testing all these steps, the authors prepared the fat tissue using the best method chosen after the first analysis to test cell differentiation (adipogenesis, osteogenesis, and chondrogenesis) and proliferation. Results The analysis showed that saline solution 0.9% was the washing solution that induced less cell death. Regarding anesthetic and vasoconstrictor drugs, lidocaine, ropivacaine, and epinephrine caused similar levels of cell death, but sodium bicarbonate increased cell proliferation. The number of passages through a 2.4 mm Luer-to-Luer transfer device did not influence cell viability.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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