Fatty Acids Have Different Adipogenic Differentiation Potentials in Stromal Vascular Cells Isolated from Abdominal Fat in Laying Hens.
Regassa A., Suh M., Datar J., Chen C., Kim WK.
Animal Study, published in Lipids (2017) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Lipids (2017)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 28523479
- DOI
- 10.1007/s11745-017-4261-2
Abstract (original English)
This study was conducted to examine the effects of fatty acids (FA) with/without chicken serum (CS) on the expression of adipogenic transcripts and adipogenesis in chicken stromal vascular cells (SVC). In experiment 1, SVC were grown in DMEM containing 10% FBS (Control) and treated with 300 µM oleic acid (OLA) + FBS, linoleic acid (LNA) + FBS, palmitic acid (PAM) + FBS, or stearic acid (STA) + FBS for 48 h. In experiment 2, cells were grown in DMEM containing 5% CS and treated with 300 µM OLA (CS + OLA), PAM (CS + PAM), STA (CS + STA) or 200 µM LNA (CS + LNA) for 48 h. Adipogenesis was determined using Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity. The proportion of OLA, PAM, or STA was increased (P < 0.05) in SVC grown in either FBS or CS with OLA, PAM or STA. Adipogenesis was induced in FBS + OLA, FBS + LNA, FBS + PAM, FBS + STA, CS + OLA, CS + LNA, CS + PAM, or CS + SAT compared to FBS. GPDH activity was significantly higher in FBS + OLA and FBS + LNA than one in FBS. Compared to FBS, the expression of FABP4 mRNA increased (P < 0.05) in FBS + OLA, FBS + LNA, or FBS + PAM, whereas that of C/EBPα, C/EBPβ, and ATGL increased (P < 0.05) in FBS + OLA or FBS + LNA cells. Expression of FABP4 and C/EBPβ mRNA was higher in CS, CS + OLA, CS + LNA, CS + PAM, or CS + SAT compared with (FBS, whereas the expression of ATGL and C/EBPα was higher in CS, CS + OLA,
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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