First comparison of commercial systems to prepare nanofat: technical performances and biological quality differ among obtained products.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Nanofat is a relatively recent fat grafting technique obtained involving the mechanical emulsification of adipose tissue whose preparation is produced at the patient's bedside. Although it was initially reported to improve skin quality in intradermal applications, it is now increasingly used in regenerative medicine. However, the absence of standardized protocols and the diversity of commercial devices result in nanofat products of variable quality. This study presents the first comprehensive comparison of nanofat obtained from different commercially available preparation systems, combining both their technical performance and biological characterization. Lipoaspirates from five healthy donors were processed using eight commercially available devices for nanofat production using emulsification or micronization techniques. The technical parameters included preparation time, ease of preparation and injection, volumetric yield, and residual aqueous fraction. Biological analyses included stromal vascular fraction isolation with evaluation of cell viability, viable nucleated cell yield, immunophenotypic cell subtype characterization and clonogenic capacity. These parameters were compared using a scoring model that enabled inter-kit ranking, integrating both a technical performance score and a biological quality score. Additionally, nanofat-conditioned media were collected for extrac

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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