Frequency of stromal lineage colony forming units in bone marrow of peroxisome proliferator-activated receptor-alpha-null mice.
Wu X., Peters JM., Gonzalez FJ., Prasad HS., Rohrer MD., Gimble JM.
Animal Study, published in Bone (2000) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Bone (2000)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 10617153
- DOI
- 10.1016/s8756-3282(99)00238-0
Abstract (original English)
The bone marrow stroma, consisting of adipocytes, fibroblasts, and osteoblasts, develops from a multipotent mesenchymal progenitor. The recently described nuclear hormone receptors, known as peroxisome proliferator-activated receptors (PPARs), regulate transcription of genes involved in adipogenesis. Consistent with this is the observation that PPARalpha-null mice exhibit greater extramedullary adipose stores compared with their wild-type controls. To determine if the status of the PPARalpha protein also influenced bone marrow stromal cell differentiation, this study compared the frequency of colony forming units for bone marrow adipocytes (CFU-A), alkaline phosphatase-positive fibroblasts (CFU-F/ALP+), and osteoblasts (CFU-O) between wild-type and PPARalpha-null mice. The CFU frequencies for all lineages were not significantly different in either gender at age 3 weeks, independent of the PPARalpha background. However, histologic analysis showed that the cross-sectional area of the femur in male PPARalpha null mice was significantly greater than that of PPARalpha-null female mice and of both wild-type genders. This was due to an increased marrow cavity space rather than an increased cortical bone area. In addition, while the percentage area of cortical bone occupied by lacunae was equivalent in the PPARalpha and wild-type males, this value was significantly greater in PPARalpha
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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