Gene profile of soluble growth factors involved in angiogenesis, in an adipose-derived stromal cell/endothelial cell co-culture, 3D gel model.
Cun X., Xie J., Lin S., Fu N., Deng S., Xie Q.
Animal Study, published in Cell Prolif (2015) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Cell Prolif (2015)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 26037311
- PMCID
- PMC6496362
- DOI
- 10.1111/cpr.12193
- Citations
- 15
Abstract (original English)
Objectives The aim of this study was to investigate gene expressions of growth factors for angiogenesis, in a three-dimensional (3D) gel populated with adipose-derived stromal cells (ASCs) and endothelial cells (ECs) in co-culture. Materials and methods The 3D gel, mixed with green fluorescent protein (GFP)-positive ASCs and DsRed-Express-positive ECs, 1:1 ratio, was established in vitro. The phenomenon of angiogenesis was observed using confocal microscopy. To detect gene expressions for growth factor proteins in both ASCs and ECs, transwell co-culture was used, and cell lysate samples were collected at 1, 3, 5 and 7 days. Semi-quantitative polymerase chain reaction (PCR) was conducted to quantify mRNA expressions of the growth factors. Results Angiogenesis was first observed in the gels by 7 days post-implantation. Over this time in ECs, genes coding for VEGFA/B, IGF-1, HIF-1α, FGF-1/-2 and BMP-5/-7 significantly increased. Meanwhile in ASCs, genes coding for VEGFA/B, IGF-1, HIF-1α, FGF-1/-2 and BMP-6 also were significantly enhanced. In particular, increased amounts of IGF-1 and HIF-1α in both ECs and ASCs were prominent relative to other factors. Conclusions Contact co-culture with ASCs and ECs at 1:1 ratio, in the 3D gel promoted angiogenesis; non-contact co-culture further confirmed gene expressions for growth factors, VEGFA/B, IGF-1, HIF-1α and FGF-1/-2 in both ASCs and
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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