The generation of brown and white adipose organoids from induced pluripotent stem cells through differentiation into a neuromesodermal stage.
Bulut-Okumuş E., Böke ÖB., Şenkal-Turhan S., Soydan HE., Sümer E., Beydilli Ö.
Animal Study, published in Life Sci (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Life Sci (2026)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42342067
- DOI
- 10.1016/j.lfs.2026.124545
Abstract (original English)
Adipose tissue is a highly heterogeneous endocrine organ with essential roles in energy storage, metabolic regulation, and thermogenesis. Here, brown adipose (BA) and white adipose (WA) organoids were generated from induced pluripotent stem cells (iPSCs) via differentiation into a neuromesodermal progenitor (NMP) state for the first time, and brown and white differentiation was confirmed by uncoupling protein 1 (UCP1) and fatty acid binding protein 4 (FABP4) expression. Morphological, histological, and ultrastructural analyses revealed depot-specific lipid droplet morphologies. Fatty-acid profiling revealed depot-like lipid signatures, and elevated triglycerides across lipid classes confirmed robust lipid accumulation in the organoids. Notably, small-molecule treatment used for obesity screening induced tissue-relevant responses in adipose organoids, highlighting their potential for drug testing. Moreover, liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics analysis revealed distinct programs associated with brown and white adipose-like states, including mitochondrial/thermogenic signatures in BA organoids and enrichment of endomembrane-trafficking pathways in WA organoids. Furthermore, transplantation into CD1 nude mice demonstrates tissue integration with enhanced vascularization of the graft. iPSC-derived adipose organoids reproduce key depot-like phenotypes
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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