Generation of hTERT-immortalized human mesenchymal stromal cells with optical and magnetic labels for in vivo transplantation and tracking
Gabashvili AN., Namestnikova DD., Gulyaev MV., Pevzner IB., Bocharnikov AD., Alexandrushkina NA.
Animal Study, published in Stem Cell Res Ther (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Stem Cell Res Ther (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41239465
- PMCID
- PMC12619327
- DOI
- 10.1186/s13287-025-04748-x
Abstract (original English)
Background Mesenchymal stem/stromal cells (MSCs) are the focus of increasing research as a potential therapeutic agent for a range of nervous system diseases, due to their unique capacity for self-renewal and differentiation. The subsequent tracking of cells post-transplantation into the organism is of pivotal significance, as it elucidates their fate, distribution, and enables the timely monitoring of any adverse effects. In the context of cell monitoring, the utilization of a non-toxic label that exhibits long-term stability is of paramount importance. Methods A human immortalized MSCs cell line was engineered to express a green fluorescent protein (GFP) and bacterial nanocompartments (high-molecular-weight icosahedral capsid-like protein complexes) via lentiviral transduction. The obtained cells were characterized by inductively coupled plasma mass spectrometry (ICP-MS) and Perls staining as well as using the nonlinear magnetization method, confocal microscopy and flow cytometry. An animal study was conducted in Sprague-Dawley rats. Results In this study, an immortalized human MSCs cell line with stable expression of a novel magnetic resonance (MR) reporter label was established for the first time. GFP was genetically produced for utilization as an optical tag. A nanocompartment of the bacterium Quasibacillus thermotolerans (Qt) was used as a carrier of the magnetic label. T
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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