GLP-1RA in JBMMSCs promoted osteogenic differentiation via modulating CREB and BRD4 signaling mediated proliferation and stemness
Liu H., Tian Y., Bao X., Li Y.
Animal Study, published in Sci Rep (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Sci Rep (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41258401
- PMCID
- PMC12630770
- DOI
- 10.1038/s41598-025-24633-0
- Citations
- 1
Abstract (original English)
This study aims to examine the molecular mechanisms underlying the curative effects of jaw bone marrow mesenchymal stem cells (JBMMSCs) on rat mandibular osteoporosis following semaglutide administration. We acquired data from rats with osteoporosis via the GEO database for bioinformatics analysis. JBMMSCs (Jaw Bone Marrow Mesenchymal Stem Cells) were then isolated from rat mandibles and characterized via flow cytometry. To evaluate the therapeutic effect of semaglutide on osteoporosis following JBMMSC intervention in osteoporotic rat models, we employed Haematoxylin and eosin (HE) staining. In vitro, the ideal semaglutide concentration for JBMMSC osteogenic induction was established using CCK-8 viability, colony formation, and scratch migration assays. JBMMSCs were subsequently injected into the tail vein for osteoporosis treatment. Protein expression related to adipogenic and osteogenic differentiation pathways was assessed by Western blotting. Furthermore, we evaluated the osteogenic (using ALP and ARS staining) and adipogenic (using Oil Red O staining) differentiation potential of JBMMSCs. Finally, to elucidate the molecular mechanisms underlying semaglutide's treatment of osteoporosis mediated by JBMMSCs, we analyzed the protein levels of key signaling molecules including p-CREB, YAP, BRD4; pluripotency factors OCT4, SOX2, c-Myc; cell cycle regulator Cyclin D1; signaling m
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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