Graphene Oxide Quantum Dots Promote Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth via the Wnt/ β -Catenin Signaling Pathway
Yang X., Zhao Q., Chen J., Liu J., Lin J., Lu J.
Laboratory Study, published in Stem Cells Int (2021) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Stem Cells Int (2021)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 33628273
- PMCID
- PMC7886518
- DOI
- 10.1155/2021/8876745
- Citations
- 13
Abstract (original English)
Graphene oxide quantum dots (GOQDs) are a carbon nanomaterial with broad potential for application in the field of nanomaterial biomedicine. Stem cells from human exfoliated deciduous teeth (SHEDs) play an important role in tissue engineering and regenerative medicine. This study investigated the effects of GOQDs on SHED osteogenic differentiation. GOQDs were synthesized; then, the proliferation of SHEDs incubated in GOQDs at different concentrations was evaluated; and the live cells were observed. We observed that live SHEDs incubated in GOQDs emitted green fluorescence in the absence of chemical dyes, and 1, 10, and 50 μ g/mL GOQDs significantly promoted SHED proliferation. Culture with the osteogenic induction medium containing 10 μ g/mL GOQDs induced calcium nodule formation, increased alkaline phosphatase (ALP) activity, and upregulated SHED mRNA and protein levels of OCN, RUNX2, COL I, and β -catenin. With the addition of Dickkopf 1 (DKK-1) or β - catenin knockdown, expression levels of the above mRNAs and proteins were decreased in GOQD-treated SHEDs. In summary, at a concentration of 10 μ g/mL, GOQDs promote SHED proliferation and osteogenic differentiation via the Wnt/ β -catenin signaling pathway. This work provides new ideas and fundamental information on interactions between GOQDs and SHEDs that are relevant for the biomedical engineering field.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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