GSTP1-modified exosomes derived from ADMSCs relieve LPS-induced acute lung injury.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Acute lung injury (ALI) is a life-threatening critical condition with high mortality. Adipose mesenchymal stem cell (ADMSC)-derived exosomes hold great potential for clinical therapy and drug delivery. Given that glutathione S-transferase Pi 1 (GSTP1), a key enzyme in oxidative stress response, is highly expressed in lung tissue and has been implicated in mitigating inflammatory and oxidative damage. However, the research on GSTP1-mediated ADMSC-derived exosomes in ALI is still blank. ADMSCs were characterized using flow cytometry, oil red O, Alizarin Red S, and clone formation assays. Gene expression was detected by quantitative real-time PCR (qRT-PCR) and western blotting. Exosomes were isolated and characterized by nanoparticle tracking analysis (NTA) and transmission electron microscope (TEM), and their uptake was measured adopting the 3,3'-dioctadecyloxacarbocyanine perchlorate (DIO) probe. In vitro and in vivo ALI models were established exploiting lipopolysaccharide (LPS). Moreover, the cell viability, cytotoxicity, inflammatory response, cell apoptosis, oxidative stress markers, mitochondrial membrane potential, and mitochondrial DNA (mtDNA) and adenosine 5'-triphosphate (ATP) levels, as well as hematoxylin-eosin (H&E) staining were analyzed to evaluate the effects of GSTP1-modified exosomes on ALI. ADMSCs were positive for CD90 and CD73 and negative for CD34 and CD45.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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