Harnessing rat derived model cells to assess the toxicity of TiO 2 nanoparticles.
Sarikhani M., Vaghefi Moghaddam S., Firouzamandi M., Hejazy M., Rahimi B., Moeini H.
Animal Study, published in J Mater Sci Mater Med (2022) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Mater Sci Mater Med (2022)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 35507219
- PMCID
- PMC9068637
- DOI
- 10.1007/s10856-022-06662-7
- Citations
- 10
Abstract (original English)
Until now, a few studies have been conducted on the destructive effects of TiO 2 NPs in living organisms, and studies on the toxicity of TiO 2 NPs are still in the beginning phases. Because of the widespread use of TiO 2 NPs in all areas of human life, it is essential to study their profound and fundamental toxic effects on each organ and body cell. Herein, we evaluate the effect of exposure to TiO 2 NPs on in vitro models derived from the rat bone marrow and adipose tissues. Exposure to TiO 2 NPs at 100 and 200 μg/ml exhibited cytotoxicity for the rat bone marrow mesenchymal stem cells (rBMSCs) and rat adipose mesenchymal stem cells (rATSC), respectively. Additionally, reduced rBMSCs and rATSCs frequencies in the S phase of the cell cycle. Moreover, TiO 2 NPs enhanced the activity of cellular senescence-associated β-galactosidase in both model cells. Significantly higher relative expression of aging-related genes P53 and NF-kB (p < 0.05) and lower expression levels of anti-aging-related genes Nanog and SIRT1 were found in the treated cells (p < 0.05). Colony-forming and DAPI staining showed the reduction of cell growth and DNA damage in both rBMSCs and rATSCs. Our findings along with other similar findings showed that TiO 2 NPs probably have negative effects on the cell growth, prompt the cells for entry into proliferation stop, DNA damage, and trigger the aging process. Graph
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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