Hierarchical stabilization of bioactive hydrogels by multi-arm peptide-polymer supramolecular staples.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Supramolecular peptide hydrogels offer attractive bioactivity and dynamic mechanical behavior for three-dimensional cell culture and tissue engineering. However, their broader use is often limited by slow gelation and insufficient mechanical stability. Here, we introduce a molecular design strategy in which a tryptophan zipper pendant multiarm poly (ethylene glycol) (Trpzip-PEG) conjugate is incorporated into Trpzip nanofibrillar hydrogels to facilitate hierarchical tuning of materials properties. Trpzip peptides self-assemble into entangled nanofiber networks, while the addition of Trpzip-PEG conjugate induces reorganization of these assemblies. Electron microscopy and neutron scattering reveal more densely bundled fibers with increased microporosity and a fractal network architecture, suggesting that the conjugate acts as a supramolecular binder or "staple" coordinating nano- and micro-scale organization. These structural changes markedly accelerate gelation and increase stiffness, yield behavior, and thixotropic recovery. Importantly, the Trpzip/Trpzip-PEG supramolecular hybrid hydrogels remain cytocompatible, supporting adipose-derived stem cell adhesion, viability, and proliferation over time. Together, these findings demonstrate that Trpzip/Trpzip-PEG hybrid hydrogels offer a versatile platform for engineering mechanically robust yet bioactive soft materials for 3D cell c

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

Browse all related research

Filter the research library by this study's title keywords, author, or publication year.