Human adipose-derived stem cells enhance the survival and neuritogenesis of auditory neurons.
Schendzielorz P., Rak K., Nguyen J., Frölich K., Scherzad A., Hagen R.
Animal Study, published in Neuroreport (2015) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Neuroreport (2015)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 26204166
- DOI
- 10.1097/WNR.0000000000000427
- Citations
- 3
Abstract (original English)
To evaluate the effect of human adipose-derived stem cells (ASCs) on auditory neurons with respect to a potential application in cochlear implantation. Spiral ganglions from rats were enzymatically dissociated and cocultured with or without ASCs in a coculture system. After 24 and 48 h, inserts with ASCs were removed and the dissociated neurons were fixed and stained. Then, a cell count of all dissociated cells and neurons was performed and the length of the neurites was evaluated. The potential effect of ASCs on the direction of neurite outgrowth was evaluated in a chemotaxis assay. For this, one chamber of the assay was filled with ASCs in medium and the other chamber filled with medium only. After 48 h, the growth direction of neurite outgrowth was evaluated. Cocultivation of auditory neurons with ASCs resulted in a marked and statistically significant increase in neurite outgrowth at 24 and 48 h after plating. Moreover, the number of surviving neurons and the percentage of neurons among the dissociated cells were higher in the coculture group compared with the control group during both observation periods. However, this difference was statistically significant only at one time point. A directional outgrowth of the neurons was not observed. ASCs appear to enhance the survival and the neuritogenesis of auditory neurons. This might be of particular interest for cochlear implan
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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