Human bone marrow derived stem cell differentiation on 3D printed bioactive glass scaffolds
Li S., Mohammed AA., Nommeots-Nomm A., Shi X., Barrak F., Heyraud A.
Animal Study, published in J Mater Sci Mater Med (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Mater Sci Mater Med (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 40864299
- PMCID
- PMC12391171
- DOI
- 10.1007/s10856-025-06918-y
- Citations
- 1
Abstract (original English)
Bioactive glass particles have previously been found to stimulate new bone growth in vivo and have a long clinical track record. The effect of bioactive glasses on human bone marrow derived stromal cells (hBMSCs) has not been clearly ascertained previously. Recently, 3D printed scaffolds of the ICIE16 glass composition (49.46 mol% SiO 2 , 36.6 mol% CaO, 6.6 mol% Na 2 O, 6.6 mol% K 2 O, 1.07 mol% P 2 O 5 ) were found to produce high quality bone ingrowth in vivo in a rabbit model. This composition was chosen because it can be sintered into scaffolds without crystallisation. Here, we cultured hBMSCs on the 3D printed ICIE16 scaffolds to determine whether the scaffolds can support cell growth and osteogenic differentiation in vitro, with and without the presence of osteogenic supplements. This was compared to a control of culture media containing dissolution products of the bioactive glass scaffold. Our hypothesis was that the cells cultured on the scaffolds would undergo more osteogenic differentiation than cells cultured in media containing only the dissolution ions of the scaffolds, even without osteogenic supplements. hBMSCs cultured on ICIE16 scaffolds significantly increased expression of osteogenic differentiation and matrix formation markers, including Runx 2, Col1a1, Osteopontin, Osteocalcin and Alkaline Phosphatase, in comparison to monolayer cultures in basal conditions
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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