Human Mesenchymal Stem Cell-Derived Exosomes Promote the Proliferation and Melanogenesis of Primary Melanocytes by Attenuating the H 2 O 2 -Related Cytotoxicity in vitro
Wang Y., He Z., Luo B., Wong H., Wu L., Zhou H.
Laboratory Study, published in Clin Cosmet Investig Dermatol (2024) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Clin Cosmet Investig Dermatol (2024)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 38524392
- PMCID
- PMC10959324
- DOI
- 10.2147/ccid.s446676
- Citations
- 4
Abstract (original English)
Background Mesenchymal stem cell-derived exosomes (MSC-Exo) have therapeutic potential. However, the impact of MSC-Exo on the survival and melanogenesis of human primary melanocytes following H 2 O 2 -induced damage has not been clarified. We therefore investigated the effects of MSC-Exo on the H 2 O 2 -affected survival of human primary melanocytes and their proliferation, apoptosis, senescence, and melanogenesis in vitro. Methods MSC-Exo were prepared from human MSCs by sequential centrifugations and characterized by Transmission Electron Microscopy, Western blot and Nanoparticle Tracking Analysis. Human primary melanocytes were isolated and treated with different concentrations of MSC-Exo, followed by exposing to H 2 O 2 . Furthermore, the impact of pretreatment with MSC-Exo on the proliferation, apoptosis, senescence and melanogenesis of melanocytes were tested by CCK-8, flow cytometry, Western blot, L-Dopa staining, tyrosinase activity and RT-qPCR. Results Pretreatment with lower doses of MSC-Exo protected human primary melanocytes from the H 2 O 2 -triggered apoptosis, while pretreatment with higher doses of MSC-Exo enhanced the H 2 O 2 -induced melanocyte apoptosis. Compared with the untreated control, pretreatment with a lower dose (1 µg/mL) of MSC-Exo enhanced the proliferation of melanocytes, abrogated the H 2 O 2 -increased p53, p21, IL-1β, IL-6 and IL-8 expression a
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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