Hydrogen gas improves proliferation and mitochondrial activity of human adipose-derived stem cells after cryopreservation.
Kimura K., Tabata Y.
Laboratory Study, published in Regen Ther (2024) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Regen Ther (2024)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 39246699
- PMCID
- PMC11379669
- DOI
- 10.1016/j.reth.2024.08.004
- Citations
- 2
Abstract (original English)
The objective of this study is to evaluate the effect of hydrogen gas on the biological functions of human adipose-derived stem cells (hADSC) in cryopreservation. hADSC were cryopreserved by a commercial cell preservation solution in the presence of hydrogen gas. After cryopreservation at -80 °C, the viability, initial attachment morphology, and biological parameters of cells cryopreserved were evaluated to compare with those of cells cryopreserved in the absence of hydrogen gas. The hydrogen concentration in the cell preservation solution was 2.0 ppm immediately after preparation and after that decreased with time. The presence of hydrogen gas permitted cells to significantly increase the proliferation of cells in addition to the percent initial adhesion. The number of cells in the spread state was significantly high compared with that of hydrogen gas-free cryopreserved cells. The cell cycle measurement with the flow cytometry and measurement of intracellular reactive oxygen species (ROS) were performed to demonstrate an enhanced cell cycle and a decreased ROS production. In the cell cycle assay, the percentage of cells in the mitotic phase increased. The presence of hydrogen gas decreased hydroxyl radicals immediately to a significantly great extent after thawing. It is concluded that the presence of hydrogen gas during cryopreservation is promising to improve the biological
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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