Hypoxia-Preconditioned Adipose Stem Cell Exosomes Promote Adipose Graft Vascular Regeneration via miRNA-126.
Song J., Hou M., Guo Y., Peng H., Liu S., Zhu J.
Animal Study, published in Aesthetic Plast Surg (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Aesthetic Plast Surg (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 39762395
- DOI
- 10.1007/s00266-024-04645-3
- Citations
- 3
Abstract (original English)
Adipose-derived mesenchymal stem cell-derived extracellular vesicles (ASCs-Exos) possess angiogenic potential, which can enhance the retention rate of fat grafts. Hypoxic preconditioning can augment their functionality. However, the optimal conditions for hypoxic preconditioning and the specific mechanisms by which it exerts its effects are not well defined. This study aimed to screen for the optimal hypoxic culture conditions for ASCs and to preliminarily elucidate the mechanisms by which they exert their effects. Flow cytometry and TUNEL fluorescence staining were employed to determine the optimal oxygen concentration and culture duration for hypoxic preconditioning of ASCs-Exos. Subsequently, a mixture of human adipose tissue and hypoxic preconditioned ASCs-Exos was implanted into the subcutaneous fat of nude mice. At 4 and 12 weeks post-transplantation, the fat graft specimens were weighed, and hematoxylin and eosin (H&E) staining and immunohistochemistry were performed to examine the samples. The expression of CD31 was assessed to analyze the formation of neovascularization. The expression of miRNA in Exos was then detected, and the influence of ASCs-Exos derived miRNA-126 on the proliferative capacity of neovascularization was verified through an EDU fluorescence assay. ASCs-Exos exhibited optimal performance when cultured under an oxygen concentration of 5% for 24 h. Aft
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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