<i>Lactobacillus brevis</i>-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins
Cho YH., Kim JW., Kim N., Kim HS., Jang JH., Bae JT.
Laboratory Study, published in Clin Cosmet Investig Dermatol (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Clin Cosmet Investig Dermatol (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 40356846
- PMCID
- PMC12068317
- DOI
- 10.2147/ccid.s512793
- Citations
- 4
Abstract (original English)
Introduction The human skin, comprising the epidermis, dermis, and subcutaneous fat layers, serves as a critical barrier against external stimuli. The integrity of this barrier function is essential for preventing skin damage and diseases. When compromised, it can lead to various dermatological issues. Methods This study investigated the efficacy of Lactobacillus brevis J2K55-derived exosomes (LBDEs) on enhancing skin barrier function. High-purity LBDEs were produced and characterized using nanoparticle tracking analysis and Cryo-TEM, concentrated to 1.52×10 8 particles/mL with sizes ranging from 50 to 200 nm. The LBDEs were then applied to human keratinocytes, HaCaT cells, and a live human skin model to analyze the expression of genes significant to skin barrier function. Results In vitro experiments demonstrated that 2.5% LBDEs increased Filaggrin mRNA expression by 301.80% compared to the control. In an ex vivo skin damage model induced by physical stimulation and UVB (Ultraviolet B) irradiation, 1% LBDEs treatment significantly upregulated the expression of key barrier-related proteins, including Aquaporin-3 (180.8%), Claudin-1 (205.4%), Filaggrin (309.9%), Loricrin (365.2%), and Serine palmitoyltransferase (191.3%), in comparison to the friction and UVB-induced control group. Conclusion These results suggest that LBDEs have potential in enhancing skin barrier function, as
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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