Immortalization of Adipose-Derived Stromal Cells From Japanese Black Cattle.
Sasaki R., Matsukura H., Meguro R., Bai L., Sawai K., Sugano E.
Animal Study, published in Cell Biol Int (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Cell Biol Int (2026)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42559951
- DOI
- 10.1002/cbin.70194
Abstract (original English)
Adipose tissue plays a critical role in determining the characteristic palatability of Japanese Black cattle beef, including its distinctive aroma, tenderness, and flavor. These quality traits are closely associated with adipogenic differentiation and lipid metabolism; however, the underlying molecular mechanisms remain poorly understood. Adipose-derived stromal cells (ASCs) residing within adipose tissue possess the capacity to differentiate into adipocytes and other mesenchymal lineages and are, therefore, considered valuable experimental resources for adipose tissue research. Nevertheless, primary cells are subject to several limitations, including a restricted proliferative lifespan caused by culture-induced stress and reduced experimental reproducibility due to inter-individual variability, which hamper their use in long-term in vitro analyses. In this study, we immortalized ASCs derived from Japanese Black cattle using the K4DT method, which involves the introduction of the R24C mutant form of cyclin-dependent kinase 4 (CDK4 R24C ), Cyclin D1, and telomerase reverse transcriptase. The established bovine ASCs (bASCs) [bASCs-K4DT] exhibited a stable proliferative capacity during long-term culture while retaining their ability to undergo adipogenic differentiation. These immortalized bASCs may serve as a useful in vitro model for elucidating the molecular mechanisms underlyi
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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