The impact of extracellular matrix proteins on bovine fibro-adipogenic progenitor cell adhesion, proliferation, and differentiation in vitro.
Gish P., Stewart M., Khuu B., Meyer N., Vahmani P., Smith L.
Animal Study, published in Physiol Rep (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Physiol Rep (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40312265
- PMCID
- PMC12045701
- DOI
- 10.14814/phy2.70283
- Citations
- 4
Abstract (original English)
Fibro-adipogenic progenitor cells (FAPs) are mesenchymal stem cells that produce extracellular matrix (ECM) and intramuscular adipocytes in skeletal muscle. While FAPs have demonstrated responsiveness to their physical environment, there is limited knowledge of how the ECM substrate of FAPs impacts their differentiation, particularly in livestock animals. We hypothesized that the ECM substrate FAPs are cultured on will differentially impact their adherence, proliferation, and differentiation. Through an initial screen of 9 ECM proteins and their combinations, significant variation of bovine FAP attachment and differentiation across coatings was observed. The ECM substrates fibronectin, collagen 6, vitronectin, and a combination of fibronectin and collagen 6 were selected for further testing. Notably, fibronectin increased cell proliferation and attachment rates, without impairing FAP adipogenic or fibrogenic differentiation compared to the other coatings. Benefits of fibronectin were maintained at lower concentrations and when combined with less favorable coatings such as collagen 6. When assessed for their adipogenic potential on each coating at different substrate stiffnesses, lipid accumulation decreased with increasing substrate stiffness, while cell attachment increased on stiffer substrates. Overall, these results demonstrate the high responsiveness of FAPs to their ECM s
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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