Improvement in nanofat preparation technology: Simple and easy-to-use adipose tissue harvesting with Liporevive.
Semina EV., Tsokolaeva ZI., Katserov DS., Prokoptsov AS., Tristanov AB., Kruglik SV.
Laboratory Study, published in JPRAS Open (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- JPRAS Open (2025)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41215837
- PMCID
- PMC12596517
- DOI
- 10.1016/j.jpra.2025.09.019
Abstract (original English)
Background Autologous adipose tissue is widely used in clinical practice as a favorable material for soft tissue filling. Despite the range of approaches for its processing the main aim is investigating a universal and simple method for processing lipoaspirate to obtain nanofat with optimal histological characteristics and cellular composition. Methods Lipoaspirates obtained from the abdominal fat were analyzed. Microfat was obtained by Luer-to-Luer Syringe adapter. Mechanical disaggregation of lipoaspirates were obtained by Liporevive equipped with stainless steel meshes. Using 30 or 50 filtrations through 1.4-, 0.8-, 0.6- and 0.4-mm pore size meshes, nanofat-containing samples were further analyzed for the presence of lipids, endothelial and stromal cells and DNA concentration. Results The Liporevive-obtained nanofat samples resembled the tissue structure of the microfat sample and revealed non-damaged parts of tissue filled with intact adipocytes. After using meshes with parameters (input/output/number of times) of 1.4/0.6/30, 1.4/0.4/30, 0.8/0.6/30, 1.4/0.6/50 and 1.4/0.4/50, nanofat samples with DNA concentrations similar to microfat were obtained. Decreasing the mesh's pore sizes to 0.4 mm (output) and increasing the number of filtrations to 50 reduced the content of fat droplets in the nanofat samples, as well as stromal, but not in the endothelial cells. Conclusion Mech
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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