Improving the Growth Rate of Human Adipose-Derived Mesenchymal Stem Cells in Alginate/Gelatin Versus Alginate Hydrogels.
Rezaei S., Shakibaie M., Kabir-Salmani M., Soltani Moghaddam M., Rezvani M., Shahali M.
Laboratory Study, published in Iran J Biotechnol (2016) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Iran J Biotechnol (2016)
- Country
- Iran
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 28959311
- PMCID
- PMC5435008
- DOI
- 10.15171/ijb.1185
- Citations
- 8
Abstract (original English)
Background Expansion and differentiation of stem cells relies on the soluble materials as well as the physical conditions of their microenvironment. Several methods have been studied in attempt to enhance the growth and differentiation rates of different adult stem cells extracted from different sources. Objectives The purpose was to improve the three-dimensional (3D) culture condition of the semi-permeable polymeric beads for encapsulation of the human adipose-derived mesenchymal stem cells (hADSCs) by modifying the ratio of the alginate-gelatin composition. Materials and methods Following isolation and characterization of hADSCs by flow cytometry and their functional differentiation, encapsulation in the alginate and alginate/gelatin compositions were performed. Moreover, the stability, swelling, size frequency, growth kinetics, and cytotoxicity of the beads were measured to meet proper condition in the designed experimental and control culture conditions. Finally, the growth rates of the cells in different experimental groups and control were measured and analyzed statistically. Results Viability decreased in 2 and 3 percent alginate once compared to 1% alginate in beads (p≤0.05). Moreover swelling of the beads in the alginate/gelatin compositions (50:50 and 70:30) were higher than the pure alginate beads (p≤0.05). Finally, the cell growth rate in alginate/gelatin (50:50) be
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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