In-vitro dissolution characteristics and human adipose stem cell response to novel borophosphate glasses.
Mishra A., Ojansivu M., Autio R., Vanhatupa S., Miettinen S., Massera J.
Laboratory Study, published in J Biomed Mater Res A (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- J Biomed Mater Res A (2019)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 31087776
- DOI
- 10.1002/jbm.a.36722
- Citations
- 1
Abstract (original English)
The main drawbacks of traditional silicate bioactive glasses are their narrow hot forming domain and noncongruent dissolution. In this article, we report on new borophosphate glasses [xM n O m + (100 - x) (47.5P 2 O 5 + 2.5B 2 O 3 + 10Na 2 O + 20CaO + 20SrO)], M n O m being CuO, Ag 2 O, and CeO 2 , having high thermal processability, hence suitable for fiber drawing and sintering into scaffolds. Furthermore, the glasses dissolve congruently in simulated body fluid (SBF) and TRIS buffer solution, eventually leading to the precipitation of a reactive layer. Human adipose stem cells (hASC) were cultured in media enriched with glass extract at different dilutions, to investigate the optimal ion concentration for cell survival. Cells grew in all the extracts, except in the undiluted Cu-doped glass extract. At dilution 1:10, the lactate dehydrogenase (LDH) activity and cell proliferation were comparable to the control, while at 1:100, the cells proliferated faster than the control. Thus, the reference (undoped), Ag and Ce-doped glasses were found to be suitable for cell viability and proliferation. Cytotoxicity assessments using the LDH assay indeed revealed the high cytotoxicity of the Cu extract. This raises questions about the use of Cu in bioactive glasses and its optimal concentration as a dopant.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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