Induction of bone formation in abdominal implants constituted by collagen sponges embedded with plant-based human transforming growth factor family proteins in ectopic dog model
Jacinto-Tinajero JC., Ascencio D., Marquina B., Barrios-Payán J., Gutierrez MC., Lim MG.
Laboratory Study, published in J Exp Orthop (2014) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- J Exp Orthop (2014)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 26914756
- PMCID
- PMC4648846
- DOI
- 10.1186/s40634-014-0011-z
- Citations
- 7
Abstract (original English)
Background Trauma, osteomyelitis, bone tumour resections and congenital deformities are the main causes of bone deficiency in which autologous graft is the preferred treatment, but usually the bone supplies are limited. Methods An experimental model of heterotopic bone formation in the subcutaneous abdominal area of dogs was developed. This model consists in omentum wrapped implants constituted by collagen type 1 sponges embedded with demineralized bone powder, calcium cloride, thrombin and platelet rich plasma; the implant is totally converted in trabecular bone after four months of implantation. This model was improved by accelerating bone production, after the isolation of the most conspicuous histological constituents (inflammatory, bone and adipose tissues) by laser microdisection and purified from them RNA that was used to determine by RT-PCR the gene expression kinetics of the most important growth bone factors. Then, the most abundant and rapidly synthesized factors were produced by genetic engineering in tobacco plants. Results Bone morphogenetic proteins 2 and 7 and transforming growth factor-β1were the most rapidly and highly synthesized factors, and they were efficiently produced in a genetic engineering plant based system in tobacco leaves. Their incorporation as recombinant proteins in the scaffold collagen sponge induced in just one month mature heterotopic bone.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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