[Influence of co-culturing vascular endothelial cells and adipose-derived stromal cells on osteogenic differentiation in vitro].
Wang F., Liu L., Zhao D., He X., Dai X., Li Y.
Animal Study, published in Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2010) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2010)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 20458998
- Citations
- 2
Abstract (original English)
To further study the influence of the co-cultivation of vascular endothelial cells (VECs) and adipose-derived stromal cells (ADSCs) on cell osteogenic differentiation in vitro and provide experimental evidences of the probability of the co-cultivation of VECs and ADSCs as the seed cells of tissue engineering. The VECs derived from cord blood and ADSCs were prepared by full-term pregnancy SD rats and 18-week-old SD rats, to carry on the morphological observation and immunohistochemical staining identification. The third generation of ADSCs and the VECs induced by conditioned medium for 6 weeks were cultured and were divided into groups A, B, and C as the experimental group according to cell ratios of 3 : 1, 1 : 1, and 1 : 3, respectively. ADSCs or VECs was cultured alone in groups D and E as control groups. ALP and alizarin red staining were done respectively on the 7th day and 14th day; ALP and osteocalcin (OC) were detected respectively on the 4th day, 7th day, and 14th day. The VECs derived from cord blood showed mixed growth of short spindle and polygonal cells after 6 weeks of induction, the immunofluorescent staining result of von Willebrand factor was positive. ADSCs showed adherent mononuclear cells and spindle-shaped growth without duplication; the immunofluorescent staining result of CD90 was positive and no positive cells were seen in the control group. On the 7th day
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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