Influence of Gold Nanoparticle Incorporation Into Glass Ionomer Cement on Fibroblast Metabolic Activity, Surface Roughness and Hardness

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objective To assess the influence of gold nanoparticle (AuNP) liquid-phase modification of glass ionomer cement (GIC) on fibroblast metabolic activity, alongside its effects on surface hardness and roughness, in an exploratory pilot in vitro study. Methods GIC specimens were prepared using liquid mixtures containing 25% and 50% AuNP suspension within the liquid phase alongside an unmodified control. The exact AuNP concentration in the final set cement was not determined, and direct structural characterisation of nanoparticle incorporation was not performed. Cell viability was evaluated using the MTT assay at 24 and 72 h and data were analysed using the Kruskal-Wallis test followed by Dunn's post hoc test. Surface hardness and surface roughness data were analysed using the Mann-Whitney U test. Significant difference was set at p Results At 24 h, the unmodified GIC group showed a mean cell viability of 3.86% ± 2.1%, while both experimental groups exhibited higher values: 15.32% ± 10.7% for the 25% AuNP-GIC group and 14.93% ± 12.6% for the 50% AuNP-GIC group. After 72 h, the 50% AuNP-GIC group maintained the highest cell viability: 8.63% ± 1.6%, which was significantly higher than the unmodified GIC group: 4.71% ± 2.6% ( p p = 0.0104). Conclusion Within the limitations of this exploratory pilot in vitro study, the 50% AuNP-containing liquid formulation showed the most favourable o

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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